Amplifiers: Polymerase Chain Reaction, Amplifier, Third-Order Intercept Point, Optical Amplifier, Parametric Oscillator, Step Resp Source Wikipedia

ISBN: 9781156940358

Published: August 12th 2011

Paperback

32 pages


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Amplifiers: Polymerase Chain Reaction, Amplifier, Third-Order Intercept Point, Optical Amplifier, Parametric Oscillator, Step Resp  by  Source Wikipedia

Amplifiers: Polymerase Chain Reaction, Amplifier, Third-Order Intercept Point, Optical Amplifier, Parametric Oscillator, Step Resp by Source Wikipedia
August 12th 2011 | Paperback | PDF, EPUB, FB2, DjVu, talking book, mp3, ZIP | 32 pages | ISBN: 9781156940358 | 7.41 Mb

Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Pages: 31. Chapters: Polymerase chain reaction, Amplifier, Third-order intercept point, Optical amplifier, ParametricMorePlease note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Pages: 31. Chapters: Polymerase chain reaction, Amplifier, Third-order intercept point, Optical amplifier, Parametric oscillator, Step response, Quantum amplifier, Multiplex ligation-dependent probe amplification, Second-order intercept point, Crest factor, Selection and amplification binding assay, Parasitic oscillation, Helicase-dependent amplification, NASBA, Polymerase cycling assembly, Touchdown polymerase chain reaction, Multiplex polymerase chain reaction, Metadyne, VOGAD, Nested polymerase chain reaction, Ligase chain reaction, Piezoelectric audio amplifier, Cycling probe technology, Servoamplifier, Intermodulation Intercept Point, Erbium-doped waveguide amplifier.

Excerpt: The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis, PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications.

These include DNA cloning for sequencing, DNA-based phylogeny, or functional analysis of genes- the diagnosis of hereditary diseases- the identification of genetic fingerprints (used in forensic sciences and paternity testing)- and the detection and diagnosis of infectious diseases. In 1993, Mullis was awarded the Nobel Prize in Chemistry along with Michael Smith for his work on PCR.

The method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplification.

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